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Cell line

Description of cell line Original reference Origin Sex & Age Source Cellosaurus (ExPaSy) Brain; U-87 MG* Glioblastoma, astrocytoma cell line: Ponten J., Macintyre EH., Acta Pathol. Microbiol. Scand. 74: 465-486 (1968) Brain: Male, 44: LGC/ATCC: CVCL_0022 Cancer cell line: U-138 MG: Glioblastoma cell line: Ponten et al., Acta Pathol Microbiol. Cell line development trends: how CHO cell line development technologies are improving biologics development from early phase to IND Table 1: ANOVA Mean Comparisons, Statistical Significance, and P/ values for A549 cell line using MTT assay data for 2,000 [micro]g/ml of Honey at 48 hrs ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose Cell line definition is - a cell culture selected for uniformity from a cell population derived from a usually homogeneous tissue source (such as an organ). How to use cell line in a sentence

Learn: cell lines - The Human Protein Atla

The cell line transcriptome was compared with the transcriptome of 37 different normal tissues and organs (Uhlén M et al, 2015).61 genes were only expressed in cell lines and not in any of the analyzed normal tissue types. These genes serve an interesting starting point to study the function and role of corresponding proteins in human biology The scientific community is increasingly recognizing that cell line integrity is critical for maintaining high standards in research. Initiatives have called for standardized cell culture quality, including confirmation of cell line identity through authentication. For years, scientists worldwide have relied on ATCC to provide fully authenticated and contamination-free biological reagents as a.

Immortalised cell lines are widely used as a simple model for more complex biological systems, for example for the analysis of the biochemistry and cell biology of mammalian (including human) cells. The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture NS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones. Concentration Vial has between 1-5 million cells as standard, however this may vary Our cell lines are extensively tested for viability, biological activity and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, thus minimizing the need for optimization or troubleshooting by the end-user

Animal cell lines and products Cell line Product Human tumour Angiogenic factor Human leucocytes Interferon Mouse fibroblasts Interferon Human Kidney Urokinase Transformed human kidney cell line, TCL-598 Single chain urokinase-type plasminogen activator (scu-PA) Human kidney cell (293) Human protein (HPC) Dog kidney Canine distemper vaccine Cow kidney Foot and Mouth disease (FMD) vaccine Chick. Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions.These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies. This cell line section provides information on the fundamentals of cell culture, including the selection of the appropriate cell line for your experiments, acquiring cell lines and the differences between adherent versus suspension culture

Cell line definition of cell line by Medical dictionar

Cell Lines Project v92, released 27-AUG-20. COSMIC, the Catalogue Of Somatic Mutations In Cancer, is the world's largest and most comprehensive resource for exploring the impact of somatic mutations in human cancer CHO Cell Lines The CHO cell line is originally derived from the Chinese hamster ovary, and has become a staple source of cells due to their robust growth as adherent cells or in suspension. They are amenable to genetic modifications and methods for cell transfection, recombinant protein expression, and clone selection are well characterized Cell Line. Cell lines used include HeLa, HEp-2, Vero, and others, and cellular responses in the presence of the toxin range from morphological changes (subjective), to more specific, e.g., metabolic status of the cells and detection of lactate dehydrogenase release from damaged cells A cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. Lines differ from cell strains in that they become immortalized. Cell culture and cell lines have assumed an important role in studying physiological, pathophysiological and the differentiation processes of specific cells The search offers you access to our complete catalogue of DSMZ human and animal cell lines. New accessions are listed here; a complete list of cell lines from our collection is also available for download.. Mycoplasma PCR assay internal and positive control DNAs are available sending the order form via fax or e-mail to the specified number or address, respectively

ATCC Cell Lines

  1. Human primary cells and immortal cell lines: differences and advantages. In research and drug development, scientists routinely use cell lines as a model for healthy or diseased tissue. Cell lines offer an easy, inexpensive and stable platform. However, they often do not fully represent what is occurring in vivo
  2. LINE is a new communication app which allows you to make FREE voice calls and send FREE messages whenever and wherever you are, 24 hours a day
  3. Human cell lines are immortalized cells propagated in vitro from primary explants of human tissue or body fluid. The use of human cell cultures as a model for more complex biological systems is an integral part of molecular biology, and biomedical research

Cell Line Definition of Cell Line by Merriam-Webste

  1. To create a cell array with a specified size, use the cell function, described below.. You can use cell to preallocate a cell array to which you assign data later.cell also converts certain types of Java ®, .NET, and Python ® data structures to cell arrays of equivalent MATLAB ® objects
  2. Quantitative proteomes of 375 cancer cell lines for the Cancer Cell Line Encyclopedia (CCLE) reveal correlated protein expression between multiple pathways and complexes as well as associations between protein complexes and genetic features including microsatellite instability, individual gene mutations, and sensitivity to gene knockdowns
  3. Cell lines are formed of cells that are able to grow indefinitely if the right conditions are met, and these can be from various sources. These cell lines are widely used in the laboratory, from.

Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting with the first successful immortal cell line HeLa, the number of available cell lines has since diversified into a plethora of options Ready-made cell lines mean you don't need to spend months generating a KO cell line to get the results you need. With our large range of ready-made knockouts in commonly used cell lines and our ever-expanding range of new targets and cell lines, obtaining your KO cell line of choice is as easy as buying a reagent Cell Line Characterization Identity - a required test to confirm the species of the origin and one of the following methods must be performed on a MCB and EOP/CAL Genetic Stability - the assessment of the cell line across the production process and is typically performed using cells from MC B to compare cells from the EOPC/CAL Finite cell lines are subjected to a definite number of passages (20 - 30 passages). Upon completion of the required number of passages, the cell line enters a stage of senescence. These cells are easy to handle as their growth is definite. The continuous cell lines are also referred to as immortalized cell lines Cell Lines The most commonly used Drosophila cell line is probably the Schneider 2 (S2) cell line, which was derived from embryos. Another popular choice for screening at the DRSC-FGR is the S2R+ cell line. But there are many other options. What cells you choose will affect

Custom Stable Cell Lines. AcceGen provides a high quality and a cost-effective service for creating a cell line expressing your gene of interest within a fast turnaround time. Several options are used for the generation of a stable cell line depending on the scope of the experiment Login. © 2020 The Broad Institute of MIT & Harvar In the previous blog post we successfully solved the problem of Excel not printing gridlines.Today I'd like to dwell on another issue related to Excel grid lines. In this article you'll learn how to show gridlines in an entire worksheet or in certain cells only, and how to hide lines by changing cells background or borders' color CRISPR-based engineered cell lines can accelerate potential treatment targets for SARS-CoV-2. Download PDF Copy; Reviewed by Emily Henderson, B.Sc. Oct 16 2020

Cell line (Science: cell culture) a cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. lines differ from cell strains in that they have escaped the Hayflick limit and become immortalised Most microglial cell lines were made before we knew much about what makes microglia unique relative to other macrophages. Arguably, the bar for microglial cell line generation — i.e., to what extent does a new microglial line recapitulate in vivo microglia? — is higher now than it was in the 1990s because we know so much more about microglia The HeLa cell line gave them the time and the possibility to conduct repeatable experiments on human cells, without testing directly on humans. And to this day, HeLa cells have saved countless lives, and many scientific landmarks (such as cloning, gene mapping, in vitro fertilization, the polio vaccine) have used HeLa cells and owe everything to the life and death of Henrietta Lacks

Interested in rapid and efficient stable cell pool/cell line development for large-scale production of your recombinant antibodies and proteins? In this sess.. Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical role in scientific advancements, yet researchers have become increasingly cautious when interpreting data generated from cell lines only

The human cell in cell line - The Human Protein Atla

Cell lineとは?エイズ用語。 Cell line【概要】 実験室の試験管の中で、人工的に維持される不死化した細胞。通常は一種の癌細胞。医学・生物学な研究~産業に欠かせない材料となっている。 【詳しく】 生きた細胞を試験管の中で飼育.. Cell line development is preparing to let go of its conventional, labor-intensive ways so that it may embrace increasingly powerful and precise optimization methods Several human acute myeloid leukemia cell lines were recently established. These lines provide model systems to study the control of differentiation in human myelogenous leukemia and, in a broader framework, the controls of normal myeloid development. The K562 line is composed of undifferentiated bl Cell line derived xenograft, CDX models are used to conduct in vivo efficacy evaluation of potential cancer therapies. Cell line derived tumor xenografts are implanted into mouse models to evaluate clients' therapeutics against clinically relevant tumor types Fetal cell lines can be put through more passages than adult cells would and they are less prone to cell aging and senescence (when cells in a culture no longer divide and start dying off)

ATCC Human Cell Lines

Immortalised cell line - Wikipedi

Creating cell lines with future manufacturability, cost of goods and speed in mind is of critical importance in today's biopharmaceutical development. By working with our cell culture teams in our Slough (UK), Visp (CH), and Tuas (SG) sites, you will benefit from a recognized and stable cell line using our GS Xceed® Gene Expression System Lymphoid Cell Line and Immunity . Lymphoid lineage cells make up about 15% of the cells in healthy bone marrow. Hematopoietic stem cells are continuously differentiating into lymphoid progenitor cells which in turn continually differentiate into the mature cells which make up the lymphoid cell line 293T cell line has its advantages like fast growth speed and high transfection efficienc ,so we ues it much in our experiment ,but I don't know the specificity of U2OS cell line.As many cell line. Our oncology cell lines cover a range of cancer models including breast, colorectal, prostate, lung, lymphoma and many more. All of our bioluminescent oncology cell lines meet the highest quality standards and are confirmed pathogen free. For research use only. Not for use in diagnostic procedures Our cell lines are available through Coriell as the Allen Cell Collection and the plasmids are available from Addgene. For ease of access, links to both of these vendors are available in the catalog pages. Internet Explorer is not supported- please use Edge, Chrome, Firefox, or Safari. Home

Cell Line Normal cells usually divide only a limited number of times before losing their ability to proliferate, which is a genetically determined event known as senescence; these cell lines are known as finite. However, some cell lines become immortal through a process calle Publisher of over 50 scientific journals across the life, physical, earth, and health sciences, both independently and in partnership with scientific societies including Cell, Neuron, Immunity, Current Biology, AJHG, and the Trends Journals HEK-293 cells are useful for many transfection experiments, particularly the propagation of adenoviral-based and retroviral-based vectors. Origins of the HEK293 Cell Line. HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells HT29 cell line and some derived cell lines thereof are of interest to study food component absorption, especially when used in co-culture with other epithelial cell lines. Although the quantitative results can be questioned in comparison with those obtained in vivo,.

Define cell line. cell line synonyms, cell line pronunciation, cell line translation, English dictionary definition of cell line. n. A cell culture that is derived from one cell or set of cells of the same type and in which under certain conditions the cells proliferate indefinitely in.. While primary cells that replace immortalized lines are more tedious to rely on because you must continue to return to an animal, they still can provide the opportunity for massive expansion of cells, which reduces the number of animals in research and allows for researchers to do basic science studies quickly, efficiently and effectively without compromising validity (Dark background indicates cell lines that are not associated with any microarrays) Show/Hide Help: Created for Radiation Oncology Branch @ NCI 2020. For any suggestions or technical difficulties with this site please contact Dr. Uma Shankavaram: Disclaimer NIHTurning Discovery Into Health® U.S. Taconic Biosciences' Syngeneic Cell Line Reference Database: use the database of published references to help you identify tumor cell lines and associated background strains in selecting the most appropriate model for your study Cell lines are still a tool of choice for many fields of biomedical research, including oncology. Although cancer is a very complex disease, many discoveries have been made using monocultures of established cell lines. Therefore, the proper use of in vitro models is crucial to enhance our understanding of cancer. Therapeutics against renal cell cancer (RCC) are also screened with the use of.

The Free Cells: The four piles in the upper left corner. The Foundations: The four piles in the upper right corner. The Tableau: The eight piles that make up the main table. The setup. The Tableau piles are numbered from 1 to 8, piles 1-4 start with 7 cards each, piles 5-8 with 6 cards each Melan-a cell line with use to investigate melanoma skin cancer. Background and Research Application Skin cancers are skin growths with differing causes and varying degrees of malignancy. The three most common malignant skin cancers are basal cell carcinoma, squamous cell carcinoma, and melanoma Recombinant stable cell line development at GenScript includes over-expression, inducible expressionI. In addition, we developed lentivirus based CRISPR/Cas (Lenti-CRISPR/Cas9) technologies and platforms, in order to meet the increasing need of knock out cell line engineering of GOI, in particular, difficult-to-transfect cancer cell lines

Cytology

NS0 Cell Line Cell Lines - Ximbi

Altogen Labs offers several stable cell line generation services: protein overexpressing cell lines, RNAi knockdown cell lines, and reporter stable cell lines (luciferase, GFP, RFP, YFP). Company scientists provide expert services with years of cell culture experience with more than 150 cancer cell lines (available in-house) There are several DC-like human cell lines that have been used for various experiments. Each has their own deficiencies and as with any immortalized or transformed line there are always questions. Proper cell line characterization assays are crucial to confirm the certainty of contaminant-free cell banks. Extensive cell line authentication, including phenotypic or genotypic identity testing, is required for mammalian, microbial, and insect cell substrates testing when working to generate biopharmaceuticals

Cell lines synonyms, Cell lines pronunciation, Cell lines translation, English dictionary definition of Cell lines. n. A cell culture that is derived from one cell or set of cells of the same type and in which under certain conditions the cells proliferate indefinitely in. Cell line engineering services Delivering exceptional value across the drug discovery pipeline Trusted expertise and proven technology. Creating your own CRISPR-Cas9 edited custom cell line model can require significant investment in both lab and human resources. Horizon has the experience and expertise to offer an.

Cell lines such as HEK293 cells are crucial for areas such as biotechnology, fundamental research into cell biology, and biomedical therapies of the future. Typically, the description of a 'cell line' is reserved for immortalized mammalian cells that can be continually cultured for a time of weeks to months without deterioration, with some well-characterized cell lines even having been. Stable Cell Lines are important tools for protein production, target validation, and functional assays. Abeomics portfolio of stable cell line includes molecules involved in immune modulation, such as, PD-1 cell line, PD-L1 cell line, PD-L2 cell line, CTLA-4 Cell line, ICOS and ICOSL Cell line, TIM3 cell line, Vista cell line etc These cell lines' ability to achieve both high cell density and high specific productivity enabled Just-Evotec Biologics to implement an intensified low-cost manufacturing processes as part of. However, an old cell-line was involved in a confirmatory tes Cell line • A cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space • A cell culture developed from a single cell and therefore consisting of cells with a uniform genetic make-up • There is presence of several cell linkage either similar or distinct • Generally stem cells are used in this cultur

Quia - Cell Division Vocabulary

Collection of Reporter Cell Lines from InvivoGe

The GeneHero™ Cas9 stable cell lines are available pre-made in many human, mouse, and rat cell line backgrounds. The S. pyogenes CRISPR Cas9 nuclease is stably integrated into either the human AAVS1 or mouse ROSA26 Safe Harbor sites, or randomly via lentiviral-mediated transduction established neuroblastoma cell lines varies (see Biological features) with cells having a neuroblastic, intermediate and substrate-adherant or flat morphology (9, 10). Cultures may be homogeneous or heterogeneous for these cell types. Reports of the adaptation of neuroblastoma cell lines to serum-fre Welcome to Human T-ALL Cell Lines database. This site collects published information about human T-cell Acute Lymphoblastic Leukemia (T-ALL) and Lymphoma cell lines, with links to source manuscripts and other web resources. We have compiled data for both commonly- & infrequently-used human T-ALL cell lines. The Table of Contents below summarizes the components of thi Stable cell lines are important in vitro cellular tools. Over-expressing cell lines are needed to screen for hits, while functional cell lines are needed for lead identification. Since 2009, GenScript has successfully generated more than 200 stable cell lines for research in this field

Highlights. The use of aborted fetus cell lines in vaccine development is well documented. In the past, at least 3 vaccines had been developed using the fetal cell lines method Tesla's pilot battery cell production line in Fremont, CA may be built in a site that spans less than 200,000 square feet, but it is poised to be one of the world's largest battery plants by.

Cell lines - SlideShar

Our cell line development timelines and innovation are unprecedented. This is our focus, which is why we do it best. Ultimately, we help companies get drugs to patients faster and more cost-effectively Cell lines with a percent match of 56-80 % are probably unrelated, but this range includes a small number of related cell lines, and further data may be needed to confirm or refute this conclusion. This matching algorithm can be used to define cell lines as unique, authenticated, misidentified, or cross-contaminated Stem Cells; iPS Cell Lines; In order to save time and eliminate the need to generate iPSCs for experimentation, we offer ready to use footprint-free iPS cell lines. These cells are completely validated and suitable for directed differentiation. They can serve as an excellent starting point for experiments, or as a control for in lab iPSC. Thus, Brehany said, while the company has some association with the use of cell lines from elective abortions, it is not responsible for that use, and its vaccine was not produced using those HEK.

Cell culture - Wikipedi

To start a new line of text or add spacing between lines or paragraphs of text in a worksheet cell, press Alt+Enter to insert a line break. Double-click the cell in which you want to insert a line break Count line breaks in cell First, the LEN function counts total characters in the cell B5. Next SUBSTITUTE removes all line returns from the text in B5 by looking for CHAR(10) which is the character code for the return character in Windows The Human Embryonic Kidney 293 (HEK293) cell line is a predominant host for both stable expression and transient expression of various research grade proteins and protein therapeutics, and more recently, five therapeutic agents produced in HEK293 cells have been approved by the FDA or the European Medicines Agency (EMA) for therapeutic use All our cell lines are certified mycoplasma free. Moreover, our parental cell lines are authenticated with short tandem repeat (STR) profiling, in accordance with recommendations by the NIH as well as several other funding organizations and major publishers*. *It has been estimated that ~18-36% of cell lines utilized in biomedical research are.

Cell Lines Thermo Fisher Scientific - U

Stable Cell Line Construction Service. To create stable cell lines is a key method in reducing the production costs of biopharmaceuticals. We use a modified FRT/Flp-In recombinase system to produce human proteins in CHO cells and 293 cells for stable expression Wu F et al. Generation of a Junctophilin-2 homozygous knockout human embryonic stem cell line (WAe009-A-36) by an episomal vector-based CRISPR/Cas9 system. Stem cell research. 2020 Oct;48:101930. Wu X et al. Generation of a human embryonic stem cell line (WAe009-A-43) carrying a Long QT syndrome related mutation in KCNH2 gene Focused Panels of Target-Relevant Cell Lines. Choosing the correct cell lines to use for in vitro evaluation of your novel agents is fast and easy with our comprehensive services.. Working with CrownBio, you gain instant access to Xeno Base ®, the world's largest commercial database of well-characterized cell lines with standard of care and RNAseq data to facilitate your selection C6 cell line, told FDA hearings in 2001 that the cell line was developed from retinal tissue from an 18 week old preborn baby. I isolated retina from a fetus, from a healthy fetus as far as. HeLa-celler är celler som isolerats från en livmoderhalscancer och som används över hela världen vid cellbiologisk, virologisk, molekylärbiologisk inte minst medicinsk forskning. De var de första mänskliga celler från vilka en permanent odödlig cellinje skapats och som förökar sig obegränsat i en cellodling.De har fått sitt namn efter den 31-åriga patient, Henrietta Lacks, från.

The GenePrint® 10 System provides a convenient STR-based method for cell line identity confirmation and for detection of intraspecies contamination in cultured human cell lines.. GenePrint® 10 coamplifies and detects nine human loci (TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317, D5S818 and D21S11).These loci collectively provide a genetic profile with a random match. Human APP (Amyloid Precursor Protein) knockout HEK293T cell line. Application: ICC, WB. Human CD47 knockout HEK293T cell line (ab266324) Description: Human CD47 knockout HEK293T cell line. Application: WB. Human EGFR knockout HeLa cell line (ab255385) Description The Cell Line Nucleofector™ Kit V is for transfection of cell lines, e.g. HepG2, HL-60, Jurkat, K-562 , MCF7, SH-SY5Y, or THP-1 The producer cell line is a key component of any GMP manufacturing campaign for a gene therapy vector. The characteristics of the selected cell line (origin/derivation, doubling time, permissiveness for viral infection and replication) determine the efficiency of viral productivity, while the growth conditions determine the requisite downstream processing methods and release tests for the. Efficient High-Quality Cell Lines. An efficient high-quality cell line is the critical first step in any biopharmaceutical manufacturing process. Achieving such a cell line can be a demanding task. It requires precise craftsmanship, requiring both expertise and hands-on experience in process development as well as reliable technology platforms

As blood cells develop, different families branch off from blood-forming stem cells. The myeloid cell line represents one such family. Cells in the myeloid cell line are those that arise from myeloid progenitor cells, and will eventually become the specific adult blood cells, shown here cell line 将不饱满个人工作量的职能事务合并,达到消减人员,提高效率的目的。也裁减了行政职能组织及其所带来的沉员----组织扁平化。也减少了搬运、空间占用,缩短了生产周期,减少搬运工具和键整嫌翻库存。 并杜绝了订单品种和数量高低起伏变化所带来的产能不足与过剩的损失,实现消减. The cell line development service global market is expected to reach $1,702.2 million by 2028 growing at a double digit CAGR from 2020 to 2028. Increasing demand for monoclonal antibodies,. Establishing Stable Cell Lines There are many different approaches for establishing stable cell lines, depending on the type of expression you're interested in (inducible vs. constitutive) and the construct that you are incorporating. This protocol is specific for the establishment of cell lines that constitutively express GFP-tagged proteins This week, President Donald Trump extolled the cutting-edge coronavirus treatments he received as miracles coming down from God. If that's true, then God employs cell lines derived from.

Mammalian Cell Lines Sigma-Aldric

HMC-1 cell line; Background Information: HMC-1 cell line was established from a patient with mast cell leukemia. HMC-1 cells are widely used in studies of human mast cell function because they exhibit many key characteristics of tissue mast cells, such as expression of histamine, tryptase, heparin and similar cell surface antigen-profile (1,2) Created from retinal tissue of 18 week gestation aborted fetus Developed in 1985, PER C6 is the growth medium for a wide variety of human disease-causing viruses that can be processed into inactivated whole virus, live-attenuated, live-vector, split, subunit and recombinant vaccines. August 12, 2002 Med-Immune Inc. To Produce New Flu Vaccine Using Cancer-Potential Aborted WIKIMEDIA, JENSFLORIAN A widely used human cancer cell line, U87MG, was first established at Uppsala University in Sweden in 1966. But a new validation study from Bengt Westermark, who was part of the team that first created U87MG and several other glioblastoma multiforme cell lines, and colleagues finds the U87MG cells available from the cell repository ATCC are not the same as the original.

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The Cell lines browser allows you to explore the different cell lines included in the project, providing a good way to see which cell lines are included or to look at a specific cell line Line breaks in plain text; Line breaks in cell formulas; One thing is certain, you shouldn't use two cells for creating the illusion of line breaks. Adding a Line Break in Plain Text. This one is easy. While you're editing the text of a cell, you can simply press Alt+Enter (or Command+Option+Enter for Mac) to add a line break as shown below GPEx® cell line development technology is a proven technology that generates high-performance, highly stable, production cell lines. Virtually any cDNA (mAb and multigenic applications) can be packaged into the GPEx® retrovector and used to insert your gene of interest into cells

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